Yeast genome Sc2.0 project aims to build the world first eukaryotic organism from scratch. While this project can help us to understand the modern organism on a whole new level, it also promotes the potential applications in many fields, such as biomanufacture and bio-medicine.
Synthetic genomics allows the redesign of life based on our understanding and intention, In 2011, researchers from US, China, UK, Singapore and Australia together innitiated the internationsl collaboration project Yeast Sc2.0. As one of the three Chinese groups in this community, BGI deeply involed in this project. In 2017, the community finished the design and synthesis of five chromosomes of yeast, and published as a special issue and cover story of Science. Several fundamental strategies for chromosome construction and Omics analysis were established. In 2018, the community published 7 papers as the second special issue of Nature Communications and showed various applications based on synthetic yeast.
1.Shen Y, Wang Y, Chen T, et al. Deep
functional analysis of synII, a 770-kilobase synthetic yeast chromosome.
Science, 2017, 355(6329).
2.Zhang W, Zhao G, Luo Z, et al. Engineering
the ribosomal DNA in a megabase synthetic chromosome. Science, 2017, 355(6329):
3.Mercy G, Mozziconacci J, Scolari V F, et
al. 3D organization of synthetic and scrambled chromosomes. Science, 2017,
4.Mitchell L A, Wang A, Stracquadanio G, et
al. Synthesis, debugging, and effects of synthetic chromosome consolidation:
synVI and beyond. Science, 2017, 355(6329).
5.Richardson S M, Mitchell L A,
Stracquadanio G, et al. Design of a synthetic yeast genome. Science, 2017,
6.Wu Y, Li B, Zhao M, et al. Bug mapping and
fitness testing of chemically synthesized chromosome X. Science, 2017,
7.Xie Z X, Li B, Mitchell L A, et al.
“Perfect” designer chromosome V and behavior of a ring derivative. Science,
8.Luo Z, Wang L, Wang Y, et al. Identifying
and characterizing SCRaMbLEd synthetic yeast using ReSCuES.[J]. Nature
Communications, 2018, 9(1).
9.Liu, W., Luo, Z., Wang, Y., et al. (2018).
Rapid pathway prototyping and engineering using in vitro and in vivo synthetic
genome SCRaMbLE-in methods. Nature communications, 9(1), 1936.
10.Jia B, Wu Y, Li B, et al. Precise control
of SCRaMbLE in synthetic haploid and diploid yeast. Nature Communications,
2018, 9(1): 1933.
11.Blount B A, Gowers G F, Ho J C, et al.
Rapid host strain improvement by in vivo rearrangement of a synthetic yeast
chromosome. Nature Communications, 2018, 9(1): 1932.
12.Shen M J, Wu Y, Yang K, et al.
Heterozygous diploid and interspecies SCRaMbLEing. Nature Communications, 2018,
13.Wu Y, Zhu R Y, Mitchell L A, et al. In
vitro DNA SCRaMbLE. Nature Communications, 2018, 9(1).
14.Hochrein L, Mitchell L A, Schulz K, et
al. L-SCRaMbLE as a tool for light-controlled Cre-mediated recombination in
yeast. Nature Communications, 2018, 9(1).
15.Shen Y, Stracquadanio G, Wang Y, et al.
SCRaMbLE generates designed combinatorial stochastic diversity in synthetic
chromosomes. Genome Research, 2016, 26(1): 36-49.