DNA methylation is one of the important epigenetic mechanisms. It is essential to know every methylated cytosine in the whole genome for understanding temporal and spatial gene expression and chromatin remodeling. MGIEasy Whole Genome Bisulfite Sequencing Library Prep Kit is specifically designed for preparing WGBS libraries for MGI High-throughput sequencing platform series. This library prep kit is optimized to convert 10-100ng fragmented DNA into a sequencing library for MGI High-throughput sequencing platforms.
Application：Platform：
DNBSEQ-G400/BGISEQ-500Reaction/kit：
16·RXN/Kit
MGIEasy Exome Capture V4 Probe Set is the first exome capture product designed and synthesized by MGI. CCDS、GENCODE、RefSeq、miRBase database are used for the probe design. As a universal exome capture kit, it is compatible with MGISEQ/BGISEQ and other high-throughput sequencing platforms.
Application：Platform：
MGISEQ/BGISEQ and other NGS platformsReaction/kit：
16·RXN/Kit
MGIEasy Exome Capture V5 Probe Set not only covers the regions of traditional exome probes, but also ensures the comprehensive capture of coding sequences related to various diseases by targeted design, e.g. Reproductive, neonatal, cardiovascular and cerebrovascular, hereditary tumors/deafness, monogenic, medication safety, personal genome, immunodeficiency, mitochondrial defects etc.
Application：Platform：
MGISEQ/BGISEQ and other NGS platformsReaction/kit：
16·RXN/Kit
The MGIEasy RAD Library Prep Kit supports library preparation for Restriction-site Associated DNA Sequence on the MGI high throughput sequencing platform. The kit provides reagents for library preparation from 1 ug of animal and plant gDNA, including restriction enzyme digestion, adaptor ligation, samples pooling, size selection, PCR, purification and single-strand circularization. It can be used in the fields of molecular marker development, genetic map construction, QTL mapping, genome-wide association analysis, population genetic analysis and molecular breeding.
Application：Molecular breeding.
Platform：
BGISEQ-500, DNBSEQ-G400
Reaction/kit：
64·RXN/Kit
The MGIEasy Universal DNA Library Prep Set is specifically designed for creating WGS libraries for the MGI high-throughput sequencing platform series. This library prep set is optimized to convert 0.5-50 ng of fragmented DNA into a customized library. This set incorporates improved Adapter ligation technology and high-fidelity PCR enzymes, which significantly increase library yield and conversion rate. All reagents provided within this set have passed stringent quality control and functional verification procedures, ensuring performance stability and reproducibility.
Application：Whole genome sequencing.
Platform：
DNBSEQ-G400、DNBSEQ-G50、BGISEQ-500、BGISEQ-50
Reaction/kit：
16 RXN / 96 RXN
The MGIEasy FS DNA Library Prep Set is specifically designed for WGS libraries construction for MGI high-throughput sequencing platform series. This library prep set is optimized to convert 5-300 ng genomic DNA into a customized library. This set uses advanced Adapter Ligation technology and High-fidelity PCR Enzymes to significantly increase library yield and conversion rate. All reagents provided within this set have passed stringent quality control and functional verification procedures, ensuring performance stability and reproducibility.
Application：Whole genome sequencing.
Platform：
DNBSEQ-G400、DNBSEQ-G50、BGISEQ-500、BGISEQ-50
Reaction/kit：
16 RXN / 96 RXN
The MGICare BRCA1/2 Sequencing Library Preparation Kit is designed for the genetic risk assessment of breast and ovarian cancer using MGI sequencing platforms. Based on optimized multiplex PCR technology and MGI high-throughput sequencing platforms, the kit is applicable for germline mutation detection in the BRCA1/2 coding region of breast/ovarian cancer susceptibility genes.
Application：Platform:
DNBSEQ-G400/DNBSEQ-G50/BGISEQ-500Reaction/kit:
96 RXN
The MGIEasy Cell-free DNA Library Prep Set is specifically designed for MGI high-throughput sequencing platforms. This library prep set is optimized to convert cell freeDNA or 150-250bp fragmented DNA into a custom library. Combined with the MGIEasy Rapid Circularization Module, PCR products are converted into circularized single strand DNA (ssCirDNA) which can be sequenced on BGISEQ/MGISEQ sequencers. All reagents provided within this kit have passed stringent quality control and functional verification procedures, ensuring performance stability and reproducibility.
Application：
MGIEasy PCR-Free Library Prep Set is specifically designed for making WGS libraries for MGI High-throughput Sequencing Platforms. This library prep set is optimized to convert 120-200 ng size selected fragmented DNA into a customized library. MGI WGS PCR-free library preparation, combined with MGI DNBSEQTM sequencer, is the true PCR-free NGS workflow without PCR amplification, resulting in no error accumulation and higher data accuracy.
Application：Platform：
DNBSEQ-G400/BGISEQ-500
Reaction/kit：
16 RXN and 96 RXN
The MGIEasy Exome FS Library Prep Set operate with gDNA followed by enzymatic fragmentation, MGI adapter ligation and amplification. The PCR product is applied to hybrid capture with MGIEasy Exome Capture Universal Set or other commercial exome capture probe. Captured product is amplified for the second time and denatured for the single stranded circle preparation which is ready for MGI high-throughput sequencing platform.
Application：Whole Exome Sequencing
Platform：
DNBSEQ-G400/DNBSEQ-G50/BGISEQ-500
Reaction/kit：
16·RXN
The MGIEasy Exome Universal Library Prep Set operate with gDNA followed by CovarisTM Focused-ultrasonicator fragmentation, MGI adapter ligation and amplification. The PCR product is applied to hybrid capture with MGIEasy Exome Capture Universal Set or other commercial exome capture probe. Captured product is amplified for the second time and denatured for the single stranded circle preparation which is ready for MGI high-throughput sequencing platform.
Application：Whole Exome Sequencing
Platform：
DNBSEQ-G400/DNBSEQ-G50/BGISEQ-500
Reaction/kit：
16·RXN
MGIEasy FS PCR-Free Library Prep Set is specifically designed for making WGS libraries without PCR for MGI High-throughput Sequencing Platforms. This library prep set could fragment 500 - 1000 ng gDNA into fragment DNA by high-quality fragmentation enzyme and prepare the library for MGI sequencing platforms. MGI WGS PCR-free library preparation, combined with MGI DNBSESQTM sequencer,is the true PCR-free NGS workflow without PCR amplification, resulting in no error accumulation and higher data accuracy.
Application：Whole genome sequencing for common species, such as human, animals, plants, microorganisms, etc.
Platform：
DNBSEQ-G400/BGISEQ-500
Reaction/kit：
16 RXN and 96 RXN
MGIEasy RNA Exome is an efficient solution for targeted quantification of the coding regions of the human transcriptome. By combining directional library prep and exome capture technologies, it involves conversion of total RNA into cDNA while keeping the strand direction information, targeted capture of exon sequences, and generation of libraries for sequencing on MGISEQ platforms. MGIEasy RNA Exome provides an efficient and complete solution for mRNA expression quantification of FFPE and other types of degraded samples. MGIEasy RNA Exome is designed specifically for degraded RNA samples, by capturing only the coding regions from total RNA, it can help improve the accuracy of mRNA quantification. It requires much less data output to achieve the same required sequencing depth for the target coding regions. MGIEasy RNA Exome Solution includes MGIEasy RNA Directional Library Prep Set, MGIEasy Exome Capture V5 Probe Set, and MGIEasy Exome Capture Accessory Kit. This guide will introduce the details of MGIEasy RNA Exome library preparation technology.
Applications:
DNA methylation is one of the important epigenetic mechanisms. It is essential to know every methylated cytosine in the whole genome for understanding temporal and spatial gene expression and chromatin remodeling. MGIEasy Whole Genome Bisulfite Sequencing Library Prep Kit is specifically designed for preparing WGBS libraries for MGI High-throughput sequencing platform series. This library prep kit is optimized to convert 10-100ng fragmented DNA into a sequencing library for MGI High-throughput sequencing platforms.
Application：Detect whole genome methylation information in single bases resolve
Platform：
DNBSEQ-G400/BGISEQ-500
Reaction/kit：
16·RXN/Kit
MGIEasy DNA Adapters-96 (Plate) kit is specifically designed for the preparation of the library of high-throughput sequencing platforms from MGI for multiplex sequencing. This kit can be used with a variety of library preparation kits. The kit contains 96 different Barcode Adapters that support up to 96 samples for batch processing of library construction and multiplex sequencing. The kit undergoes rigorous quality control and functional verification to ensure maximum stability and repeatability of library construction, as well as uniformity and accuracy of sequencing data splitting.
Application：
MGIEasy DNA Clean Beads are used for purification and size selection of DNA samples. With our optimized buffer system, DNA products more than 100bp in length can be very efficiently recovered. MGIEasy DNA Clean Beads are compatible with a variety of vendors’ DNA and RNA library construction kits. They can be used in exactly the same way as AMPure XP Beads. The PCR yield and fragment size distribution of the constructed libraries following MGIEasy DNA Clean Beads usage are consistent with those following AMPure XP Beads usage. AMPure XP Beads can be directly replaced by MGIEasy DNA Clean Beads.
Application：
MGIEasy DNA Clean Beads are used for DNA and RNA purification.
Reaction:
50 mL
Copy Number Variant (CNV) analysis has been widely applied for scientific research and clinical uses such as evolutionary research, regulation of gene expression, and reproductive genetics. Take preimplantation genetic screening (PGS) as an example. PGS is the genetic test to identify genetic defects within embryos before implantation. It helps select the best embryo for transplant to the uterus and improve the chances of successful pregnancy. However, the amount of DNA from a few cells obtained by embryo biopsy is too limited to perform this molecular analysis. Hence, PGS requires gene amplification methods to amplify scarce genomic DNA from single cell.
This kit offer solutions for single-cell DNA sequencing. It incorporates the whole genomic application (WGA) technology and next-generation sequencing (NGS) technology to analyze chromosomal abnormalities in a single cell accurately.
Detect chromosome copy number variation at the single-cell level, can be used for species evolution, reproductive development and expression regulation research.
Platform Compatibility:
DNBSEQ-G50/DNBSEQ-G400/BGISEQ-50/BGISEQ-500
Reaction/kit:
48·RXN
MGIEasy Circularization Kit is a modular library prep kit designed for MGI high-throughput sequencing platforms. This kit can be used to prepare a single-stranded circular DNA library for MGI sequencers from PCR products with MGI adapters.
All reagents provided within this kit havepassed stringent quality control and functional verification procedures to ensurehigh performance, stability, and reproducibility.
Application：This kit is applicable to PCR products with MGI adaptersfrom all MGI library prep kits. It can be used to convert a PCR product to asingle-stranded circular DNA library that can be sequenced on MGIhigh-throughput sequencing platforms.
Platform Compatibility:Sequencing instrument compatibility is dependent onspecific MGI library prep kits. Libraries created by this kit can be used onany MGI sequencer.
Reaction:16 RXN
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The MGICare Cell-free DNA Chromosomal Aneuploidy and Single gene Disease Library Prep Set is specifically designed for MGI high-throughput sequencing platforms, which can prepare WGS and 18 Single gene disease relevant target region library simultaneously, it can be applied to WGS and target region research of Cell-free DNA.
Application：Fetal chromosome aneuploidy and single gene diseasedetection and maternal single gene disease carrier screening
Platform：DNBSEQ-G400、DNBSEQ-G50、BGISEQ-500
Reaction/kit：48 RXN/kit
More
MGICare Single Gene Genomic Disease Phasing and Chromosome Copy Number Variation Sequencing Library Prep Set is specifically designed for the MGI High-throughput series sequencing platforms.
This library prep kit is optimized toprepare a whole genome-wide and Thalassemia genes region enriched library fromhuman genomic DNA and single-cell（or multiple cells）multiple displacement amplification (MDA)products. Only one library preparation is needed to detect both chromosome copynumber variations and Thalassemia gene mutation.
The kit uses multiplex PCR amplification technology combined with NGSsequencing technology and PGS/PGD integration software to accurately identifychromosomal copy number variant and thalassemia in embryos.
Application：Detection of embryonic genome chromosome copy numbervariation and whether the embryo has thalassaemia and HLA matching detection
Platform：DNBSEQ-G400、DNBSEQ-G50、BGISEQ-500
Reaction/kit：48 RXN/kit
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The MGIEasy Exome Capture V5 Universal Reagent Kit not only covers the areas covered by traditional exon probes, but also optimizes the probes to ensure full coverage of pathogenic genes related to reproductive health, newborn, cardiovascular and ce.
MGIEasy stLFR Library Prep Kit is the world's first partition-less long fragment DNA co-barcoding library prep kit. Based on the patented DNA co-barcoding technology, that is adding the same barcode sequence to sub-fragments of the original long DNA
MGIEasy exon capture V4 universal reagent kit is the first exon capture product designed based on Chinese population genome. The database for probe design is CCDS, GENCODE, RefSeq, MicroBase and so on. As a universal exon capture kit, it can be compa
APPLICATION：whole exome sequencing, covering 99% of the disease-related regions.
MGIEasy DNA rapid Library prepkit is a whole genome sequencing library construction kit for MGISEQ/BGISEQ Sequencing Platform. Using this kit, the whole genome DNA can be prepared into a single-stranded circular DNA library for MGISEQ/BGISEQ Sequenci
MGIEasy DNA rapid Library prepkit is a whole genome sequencing library construction kit for MGISEQ/BGISEQ Sequencing Platform. Using this kit, the whole genome DNA can be prepared into a single-stranded circular DNA library for MGISEQ/BGISEQ Sequenci
MGIEasy RNA Library Prep Set provides an efficient solution for generating libraries from a wide range (10 ng-1 ug) of total RNA that is suitable for MGI high-throughput sequencing platforms. It is easy to use and can be widely applied to human, ani。
MGIEasy RNA Library Prep Set provides an efficient solution for generating libraries from a wide range (0.2-2.5 μg) of total RNA that is suitable for MGI high-throughput sequencing platforms. It is easy to use and can be widely applied to human, anim
MGIEasy rRNA Depletion Kit can efficiently remove rRNA from total RNA and keep mRNA and non-coding RNA to increase the proportion of useful data. It depletes rRNA from human, mouse and rat total RNA with an input of total RNA ranging from 10 ng-1 μg.
MGIEasy Small RNA Library Prep Kit provides an efficient solution for generating libraries suitable for MGI high-throughput sequencing platforms with a broad range of input amounts (10 ng - 1 μg) of total RNA. Libraries prepared using the MGIEasy Sma